2005   Volume No 10– pages 31-39

Title: Mineralization of regenerated cellulose hydrogels induced by human bone marrow stromal cells

Authors: P. L. Granja, B. De Jéso, R. Bareille, F. Rouais, C. Baquey, M. A. Barbosa

Address: INEB - Instituto de Engenharia Biomédica, Laboratório de Biomateriais, Rua do Campo Alegre, 823 - 4150-180 Porto, Portugal

E-mail: pgranja@ineb.up.pt

Key Words: Regenerated cellulose hydrogels, human bone marrow stromal cells, cell proliferation, mineralization, calcium phosphates, hydroxyapatite

Publication date: October 10th 2005

Abstract: The proliferation of cultured human bone marrow stromal cells (HBMSC) on regenerated cellulose hydrogels was assessed. Regenerated cellulose hydrogels showed good rates of HBMSC proliferation, the cells exhibiting a flattened morphology, and after 22 days in culture, the cells had homogeneously colonized the surface of the materials. Moreover, since the early days in culture, between the surface of the materials and attached cells a continuous granulated hydroxyapatite layer was formed. It has been previously demonstrated in vitro, but without cells, that these materials did not mineralize. Hence, it seems that HBMSC promoted the mineralization of the surface.

Article download: Pages 31-39. (PDF file)