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2006 Volume No 11
pages 8-15
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Title: Do human osteoblasts grow into open-porous titanium?
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Authors: U Müller, T Imwinkelried, M Horst,
M Sievers, U Graf-Hausner
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Address: Institute of Chemistry and Biotechnology,
University of Applied Science Winterthur, Switzerland
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E-mail: muu@zhwin.ch
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Key Words: Titanium, Human osteoblasts, Proliferation,
Perfusion, Cell culture, Cage, Spine fusion, Scaffold.
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Publication date: January 19th 2006
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Abstract: A titanium foam for spine fusion and other
applications was tested by cell culture. Its high porosity
and surface roughness should enable bone cells to grow through
it, resulting in a better fixation of the vertebral body.
The foam was tested by in vitro experiments with human
osteoblasts under static culture conditions and in a perfused
system. By means of cell number, viability, scanning electron
microscopy and histological staining, cell proliferation could
be observed. The expression of osteogenic genes like collagen-I,
alkaline phosphatase and osteocalcin was proven by reverse
transcription polymerase chain reaction (RT-PCR) as well as
in the case of alkaline phosphatase with biochemical methods.
The conducted experiments showed that human osteoblasts could
grow through the interconnected porosity of the metal foam
and that they expressed an osteoblast like phenotype. The
results suggest that in vivo osteoblasts are likely
to form a trabecular bone bridge through this titanium foam.
Consequently, with this osteoconductive material, there may
be a reduced need for autologous bone in spinal fusion procedures.
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Article download: Pages
8-15 (PDF file)
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