eCM (Eur Cell Mater / e Cells & Materials) Not-for-profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2012   Volume No 23 – pages 348-361

Title: Lysophosphatidic acid-functionalised titanium as a superior surface for supporting human osteoblast (MG63) maturation

Author: JP Mansell, J Brown, JG Knapp, CFJ Faul, AW Blom

Address: Musculoskeletal Research Unit, Avon Orthopaedic Centre, School of Clinical Sciences, University of Bristol, Westbury-on-Trym, BS10 5NB, UK

E-mail: j.p.mansell at bris.ac.uk

Key Words: Titanium; surface functionalisation; covalent attachment; lysophosphatidic acid; receptor agonist; active vitamin D; osteoblast maturation

Publication date: May 9th 2012

Abstract: Covalent modifications of titanium with small molecules known to promote human osteoblast maturation are especially attractive in developing superior biomaterials. An important step in securing competent bone formation at implant sites is promoting the formation of mature osteoblasts, either from committed pre-osteoblasts or from their mesenchymal progenitors. To this end our research has focussed on identifying molecules that enhance human osteoblast formation and maturation and to develop ways of covalently attaching these molecules to implant surfaces so that they are more likely to withstand the rigors of the implantation process whilst still retaining their bioactivity. Herein we report the novel production of lipid-functionalised titanium using lysophosphatidic acid or a related compound, (3S) 1-fluoro-3-hydroxy-4-butyl-1-phosphonate. Both lipids were especially effective at co-operating with calcitriol to promote human osteoblast maturation at these modified Ti surfaces in vitro. The novel findings presented offer enticing new developments towards the fabrication of next-generation implant devices with the potential to significantly enhance the osseointegration process and with it improvements in future prosthesis performance and longevity.

Article download: Pages 348-361 (PDF file)
DOI: 10.22203/eCM.v023a27