eCM (Eur Cell Mater / e Cells & Materials) Not-for-Profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2003   Volume No 6 - pages 22-27

Title: Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies

Authors: B.D. Boyan, S. Lossdörfer, L. Wang, G. Zhao, C.H. Lohmann, D.L. Cochran & Z. Schwartz

Address: Dept. of Biomedical Engineering, Georgia Institute of Technology, Atlanta, USA

E-mail: barbara.Boyan at

Key Words: Osteoblasts, titanium, surfaces, microtopography, osteoprotegerin (OPG), osteogenesis.

Publication date: October 24th 2003

Abstract: Osteoblasts respond to microarchitectural features of their substrate. On smooth surfaces (tissue culture plastic, tissue culture glass, and titanium), the cells attach and proliferate but they exhibit relatively low expression of differentiation markers in monolayer cultures, even when confluent. When grown on microrough Ti surfaces with an average roughness (Ra) of 4-7 µm, proliferation is reduced but differentiation is enhanced and in some cases, is synergistic with the effects of surface microtopography. In addition, cells on microrough Ti substrates form hydroxyapatite in a manner that is more typical of bone than do cells cultured on smooth surfaces. Osteoblasts also respond to growth factors and cytokines in a surface-dependent manner. On rougher surfaces, the effects of regulatory factors like 1α,25(OH)2D3 or 17ß-estradiol are enhanced. The response to the surface is mediated by integrins, which signal to the cell through many of the same mechanisms used by growth factors and hormones. Studies using PEG-modified surfaces indicate that increased differentiation may be related to altered attachment to the surface. When osteoblasts are grown on surfaces with chemistries or microarchitectures that reduce cell attachment and proliferation, and enhance differentiation, the cells tend to increase production of factors like TGF- ß1 that promote osteogenesis while decreasing osteoclastic activity. Thus, on microrough Ti surface, osteoblasts create a microenvironment conducive to new bone formation.

Article download: Pages 22-27 (PDF file)
DOI: 10.22203/eCM.v006a03