2006 Volume No 11– pages 35-42
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Title: Culture medium modulates the behaviour of human
dental pulp-derived cells: Technical Note |
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Authors: S Lopez-Cazaux, G Bluteau, D Magne, B Lieubeau,
J Guicheux, B Alliot-Licht |
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Address: INSERM EM 9903, Research Center on Materials
with Biological Interest, School of Dental Surgery, 1 place
Alexis Ricordeau, 44042 Nantes cedex 1, France |
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E-mail: jerome.guicheux at nantes.inserm.fr |
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Key Words: Dental pulp, Smooth muscle actin, Odontoblast-like
cell differentiation, Cell culture |
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Publication date: February 17th 2006 |
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Abstract: In vitro approaches have extensively
been developed to study reparative dentinogenesis. While dental
pulp is a source of unidentified progenitors able to differentiate
into odontoblast-like cells, we investigated the effect of
two media; MEM (1.8mM Ca and 1mM Pi) and RPMI 1640 (0.8mM
Ca and 5mM Pi) on the behaviour of human dental pulp cells.
Our data indicate that MEM significantly increased cell proliferation
and markedly enhanced the proportion of -smooth muscle actin
positive cells, which represent a putative source of progenitors
able to give rise to odontoblast-like cells. In addition,
MEM strongly stimulated alkaline phosphatase activity and
was found to induce expression of transcripts encoding dentin
sialophosphoprotein, an odontoblastic marker, without affecting
that of parathyroid hormone/parathyroid hormone related protein-receptor
and osteonectin. In conclusion, these observations demonstrate
that not only proliferation but also differentiation into
odontoblast-like cells was induced by rich calcium and poor
phosphate medium (MEM) as compared to RPMI 1640. This study
provides important data for the determination of the optimal
culture conditions allowing odontoblast-like differentiation
in human pulp cell culture.
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Article download: Pages
35-42 (PDF file) |
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