2006 Volume No 12 – pages 44-48
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Title: Technical Note: Assay of cell quantity in the
fibroblast-populated collagen matrix with a tetrazolium reagent |
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Author: MA Carlson |
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Address: Department of Surgery, University of Nebraska
Medical Center and the Omaha VA Medical Center, Omaha, NE,
USA |
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E-mail: macarlso at unmc.edu |
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Key Words: fibroblast, collagen, matrix, lattice, wound
healing, WST-1, tetrazolium, cellular assay |
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Publication date: September 28th 2006 |
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Abstract: Determination of cell quantity in three-dimensional culture systems, such as the fibroblast-populated collagen matrix, can be difficult secondary to the abundance of extracellular collagen. The WST-1 assay, which can quantitate cellular processes in monolayer culture, was tested as a measure of relative cell quantity in a three dimensional culture system, the fibroblast-populated collagen matrix. The assay had acceptable accuracy and precision, and was not affected by the extracellular matrix. It was concluded that the assay was a reasonable alternative to other measures of cell quantity in the fibroblast-populated collagen matrix.
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Article download: Pages
44-48 (PDF file) |
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