eCM (Eur Cell Mater / e Cells & Materials) Not-for-Profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2012   Volume No 23 – pages 182-194

Title: Dynamic cell adhesion and migration on nanoscale grooved substrates

Author: E Lamers, J te Riet, M Domanski, R Luttge, CG Figdor, JGE Gardeniers, XF Walboomers, JA Jansen

Address: Department of Biomaterials, Radboud University Nijmegen Medical Centre 309 PB, PO Box 9101, 6500HB Nijmegen, The Netherlands

E-mail: j.jansen at dent.umcn.nl

Key Words: Cell-protein-material interactions, tissue-material interactions, biomaterials, nanotechnology, imaging, AFM profilometry, cells motility, cell migration, tissue adhesion

Publication date: March 13th 2012

Abstract: Organised nanotopography mimicking the natural extracellular matrix can be used to control morphology, cell motility, and differentiation. However, it is still unknown how specific cell types react with specific patterns. Both initial adhesion and preferential cell migration may be important to initiate and increase cell locomotion and coverage with cells, and thus achieve an enhanced wound healing response around an implantable material. Therefore, the aim of this study was to evaluate how MC3T3-E1 osteoblast initial adhesion and directional migration are influenced by nanogrooves with pitches ranging from 150 nm up to 1000 nm. In this study, we used a multi-patterned substrate with five different groove patterns and a smooth area with either a concentric or radial orientation. Initial cell adhesion measurements after 10 s were performed using atomic force spectroscopy-assisted single-cell force spectroscopy, and demonstrated that nascent cell adhesion was highly induced by a 600 nm pitch and reduced by a 150 nm pitch. Addition of RGD peptide significantly reduced adhesion, indicating that integrins and cell adhesive proteins (e.g. fibronectin or vitronectin) are key factors in specific cell adhesion on nanogrooved substrates. Also, cell migration was highly dependent on the groove pitch; the highest directional migration parallel to the grooves was observed on a 600 nm pitch, whereas a 150 nm pitch restrained directional cell migration. From this study, we conclude that grooves with a pitch of 600 nm may be favourable to enhance fast wound closure, thereby promoting tissue regeneration.

Article download: Pages 182-194 (PDF file)
DOI: 10.22203/eCM.v023a14