2012 Volume No 24 – pages 237-248
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Title: Multifunctional surfaces with biomimetic nanofibres and drug-eluting micro-patterns for infection control and bone tissue formation |
Author: XN Chen, YX Gu, JH Lee, WY Lee, HJ Wang |
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Address: Department of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, McLean Building Room 416, 1 Castle Point on Hudson, Hoboken, NJ 07030, USA |
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E-mail: Hongjun.Wang at stevens.edu |
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Key Words: Biomimetic nanofibres; drug-eluting micro-patterns; infection control; osteogenesis. |
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Publication date: September 24th 2012 |
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Abstract: For long-term orthopaedic implants, the creation of a surface that is repulsive to bacteria while adhesive to tissue cells represents a promising strategy to control infection. To obtain such multifunctional surfaces, two possible approaches were explored to incorporate a model antibiotic, rifampicin (Rf), into the osteogenic polycaprolactone (PCL)/chitosan (CHS) biomimetic nanofibre meshes by (1) blending Rf into the electrospinning solutions and then electrospinning into nanofibres (i.e., Rf-incorporating fibres), or (2) depositing Rf-containing poly(D,L-lactic-co-glycolic) acid (PLGA) micro-patterns onto the PCL/chitosan nanofibre meshes via ink-jet printing (i.e., Rf-eluting micro-pattern/fibre). Rapid release of Rf from both meshes was measured even though a relatively slower release rate was obtained from the Rf-eluting micro-pattern ones. Antibacterial assay with Staphylococcus epidermidis showed that both mesh surfaces could effectively kill bacteria and prevent biofilm formation. However, only Rf-eluting micro-pattern meshes favoured the attachment, spreading and metabolic activity of preosteoblasts in the cell culture study. Furthermore, the Rf-eluting micro-pattern meshes could better support the osteogenic differentiation of preosteoblasts by up-regulating the gene expression of bone markers (type I collagen and alkaline phosphatase). Clearly, compared to Rf-incorporating nanofibre meshes, Rf-eluting micro-patterns could effectively prevent biofilm formation without sacrificing the osteogenic properties of PCL/chitosan nanofibre surfaces. This finding provides an innovative avenue to design multifunctional surfaces for enhancing bone tissue formation while controlling infection. |
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Article download: Pages
237-248 (PDF file) |
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