eCM (Eur Cell Mater / e Cells & Materials) Not-for-Profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2021   Volume No 41 – pages 40-51

Title: Long term outcomes of biomaterial-mediated repair of focal cartilage defects in a large animal model

Authors: ML Sennett, JM Friedman, BS Ashley, BD Stoeckl, JM Patel, M Alini, M Cucchiarini, D Eglin, H Madry, A Mata, C Semino, MJ Stoddart, B Johnstone, FT Moutos, BT Estes, F Guilak, RL Mauck, GR Dodge

Address: 379A Stemmler Hall, 36th Street and Hamilton Walk, Philadelphia, PA 19104-6081, USA

E-mail: gdodge at pennmedicine.upenn.edu

Abstract: The repair of focal cartilage defects remains one of the foremost issues in the field of orthopaedics. Chondral defects may arise from a variety of joint pathologies and left untreated, will likely progress to osteoarthritis. Current repair techniques, such as microfracture, result in short-term clinical improvements but have poor long-term outcomes. Emerging scaffold-based repair strategies have reported superior outcomes compared to microfracture and motivate the development of new biomaterials for this purpose. In this study, unique composite implants consisting of a base porous reinforcing component (woven poly(ε-caprolactone)) infiltrated with 1 of 2 hydrogels (self-assembling peptide or thermo-gelling hyaluronan) or bone marrow aspirate were evaluated. The objective was to evaluate cartilage repair with composite scaffold treatment compared to the current standard of care (microfracture) in a translationally relevant large animal model, the Yucatan minipig. While many cartilage-repair studies have shown some success in vivo, most are short term and not clinically relevant. Informed by promising 6-week findings, a 12-month study was carried out and those results are presented here. To aid in comparisons across platforms, several structural and functionally relevant outcome measures were performed. Despite positive early findings, the long-term results indicated less than optimal structural and mechanical results with respect to cartilage repair, with all treatment groups performing worse than the standard of care. This study is important in that it brings much needed attention to the importance of performing translationally relevant long-term studies in an appropriate animal model when developing new clinical cartilage repair approaches.

Key Words: Cartilage defects, large animal models, biomaterials, scaffolds, long-term outcomes, cartilage biomechanics, tissue engineering, orthopaedics, articular cartilage repair.

Publication date: January 7th 2021

Article download: Pages 40-51 (PDF file)
DOI:
10.22203/eCM.v041a04

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